TPCN2 Antibody

• Catalog Number: orb1249458
• Category: Antibodies
• Description: TPCN2 Antibody
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-EDELTERLSQHPH
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: TPCN2
• UniProt ID: Q8NHX9
• NCBI: NP_620714.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: TPCN2, two pore segment channel 2, FLJ41094, SHEP1
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 30kDa band in Human Brain (Cerebral Cortex), Kidney and Liver lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 85.2kDa according to NP_620714.2. The 30kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Pancreas and Colon. Recommended concentration: 3.75ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249458 (3.75 ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1249458 (3.75 ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.