TPA Antibody (Center)

SKU: orb1929815

Description

TPA Antibody (Center)

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Immunology & Inflammation, Infectious Disease & Virology, Metabolism Research

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB - 1:2000
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 62917 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

TPA Antibody (Center) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

TPA Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TPA Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Anti-TPA Antibody (Center) at 1:2000 dilution + Rat plasma whole lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 63 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-TPA Antibody (Center) at 1:2000 dilution. Lane 1: Human plasma whole lysate. Lane 2: Mouse plasma whole lysate. Lane 3: Rat plasma whole lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 63 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

UniProt

P00750