You have no items in your shopping cart.
Cart summary
Item 1 of 4
Item 1 of 4
TPA Antibody (Center)
Catalog Number: orb1929815
| Catalog Number | orb1929815 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB18787 |
| Tested applications | FC, IHC-P, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:2000, WB: 1:2000, IHC-P: 1:10~50, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 62917 Da |
| Target | This TPA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 371-399 amino acids from the Central region of human TPA. |
| UniProt ID | P00750 |
| NCBI | NP_127509.1, NP_000921.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Tissue-type plasminogen activator, t-PA, t-plasmin Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
TPA Antibody (Center) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
TPA Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TPA Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Anti-TPA Antibody (Center) at 1:2000 dilution + Rat plasma whole lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 63 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-TPA Antibody (Center) at 1:2000 dilution. Lane 1: Human plasma whole lysate. Lane 2: Mouse plasma whole lysate. Lane 3: Rat plasma whole lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 63 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
