TOR1A Antibody

• Catalog Number: orb1254694
• Category: Antibodies
• Description: TOR1A Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 63-332 of human TOR1A (NP_000104.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 36kDa
• Target: TOR1A
• UniProt ID: O14656
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: Torsin-1A, Dystonia 1 protein, Torsin ATPase-1A, 3
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using TOR1A antibody (orb1254694) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded human normal breast using TOR1A antibody (orb1254694) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat liver using TOR1A antibody (orb1254694) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of A431 cells using TOR1A antibody (orb1254694) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.