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Description
Images & Validation
−
| Tested Applications | FC, ICC, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB=1:500-2000, ICC/IF=1:50-200, Flow-Cyt=1:50-100, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Human, Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | B3F3 |
| Immunogen | A synthesized peptide derived from human Topoisomerase 1 (1-45aa) |
| Target | TOP1 |
| Molecular Weight | 100 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS(pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−TOPI; D130064I21Rik; Top-1; Ab2-086; TOP1_HUMAN; TOP1; DNA topoisomerase I; 5.6.2.1; TOP1_MOUSE; TOP1_RAT; topoisomerase (DNA) I; Scl-70 antigen
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Quick Database Links
Gene Symbol
TOP1
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
IF
Immunofluorescence
ICC
Immunocytochemistry