Topoisomerase (DNA) I, Mitochondrial (TOP1MT) Antibody

• Catalog Number: orb751207
• Category: Antibodies
• Description: Mouse monoclonal antibody to TOP1MT
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: TOP1MT/613
• Tested applications: IHC, WB
• Reactivity: Human
• Isotype: IgG2b
• Immunogen: Recombinant full-length human TOP1MT protein
• Concentration: 200 μg/ml
• Dilution range: Western Blot (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 70kDa
• Target: TOP1MT
• Entrez: 116447
• UniProt ID: Q969P6
• Expression System: Bioreactor
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Alternative names: Anti-DNA topoisomerase I antibody, anti-TOP1MT ant
• Note: For research use only
• Application notes: Positive Control: A431 cells. Heart, Skeletal muscle, brain or fetal liver.
• Expiration Date: 12 months from date of receipt.