Topo IIIα rabbit pAb

• Catalog Number: orb771226
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Topo III alpha
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: Synthesized peptide derived from Topo IIIα . at AA range: 350-430
• MW: 115
• Tested applications: ELISA, WB
• Dilution range: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-TOP3A antibody, anti-TOP3 antibody, anti-DNA
• Note: For research use only

Western blot analysis of 293T using Topo IIIα antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.