TOPBP1 Antibody

• Catalog Number: orb1257329
• Category: Antibodies
• Description: TOPBP1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1253-1522 of human TOPBP1 (NP_008958.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:100 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 171kDa
• Target: TOPBP1
• UniProt ID: Q92547
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: Dpb11, TOP2BP1, DNA topoisomerase 2-binding protei
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:100 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of 293T cells, using TOPBP1 antibody (orb1257329) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 50s.

Immunohistochemistry of paraffin-embedded mouse heart using TOPBP1 antibody (orb1257329) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of A549 cells using TOPBP1 antibody (orb1257329). Blue: DAPI for nuclear staining.