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TOPBP1 Antibody
Catalog Number: orb1257329
| Catalog Number | orb1257329 |
|---|---|
| Category | Antibodies |
| Description | TOPBP1 Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IF, IHC, WB |
| Reactivity | Human, Mouse |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1253-1522 of human TOPBP1 (NP_008958.2). |
| Antibody Type | Primary Antibody |
| Concentration | batch dependent |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | Observed: 171kDa |
| Target | TOPBP1 |
| UniProt ID | Q92547 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Liquid |
| Alternative names | Dpb11, TOP2BP1, DNA topoisomerase 2-binding protei Read more... |
| Note | For research use only |
| Application notes | WB: 1:500 - 1:2000IHC: 1:100 - 1:200IF: 1:50 - 1:200 |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of 293T cells, using TOPBP1 antibody (orb1257329) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 50s.
Immunohistochemistry of paraffin-embedded mouse heart using TOPBP1 antibody (orb1257329) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using TOPBP1 antibody (orb1257329). Blue: DAPI for nuclear staining.
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