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| Catalog Number | orb685497 |
|---|---|
| Category | Antibodies |
| Description | This TOP2A Antibody is an unconjugated polyclonal product. It tagets TOP2A using a synthesized peptide derived from N-terminal of Human TOP2A as the immunogen. This antibody is suitable for ELISA, IF, IHC, WB. Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.. |
| Target | TOP2A |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | liquid |
| Buffer/Preservatives | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Immunogen | Synthesized peptide derived from N-terminal of Human TOP2A. |
| UniProt ID | P11388 |
| Tested applications | ELISA, IF, IHC, WB |
| Dilution range | WB: 1: 500-1: 3000, IHC-P: 1: 50-1: 100, IF: 1: 100-1: 500 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Anti-DNA topoisomerase 2-alpha antibody, anti-EC 5 Read more... |
| Research Area | Epigenetics & Chromatin |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |

Western blot analysis of HT-29 cells using TOP2A antibody

Immunohistochemical staining of human liver carcinoma tissu using TOP2A antibody

Immunofluorescence analysis of A549 cells using TOP2A antibody
IF, IHC-Fr, IHC-P, WB | |
Mouse, Rat | |
Human, Mouse, Rat | |
Rabbit | |
Recombinant | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IF, IHC-P, WB | |
Human, Mouse | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Human, Monkey | |
Polyclonal | |
Unconjugated |
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