TOP2A Antibody (C-term)

• Catalog Number: orb1928023
• Category: Antibodies
• Description: TOP2A Antibody (C-term)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Hamster
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: P11388
• MW: 174385 Da
• Tested applications: IF, IHC-P, WB
• Dilution range: WB - 1:2000
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: TOP2
• Research Area: Cancer Biology
• Note: For research use only

Western blot analysis of TOP2A Antibody (C-term) in A375 cell line lysates (35 ug/lane). TOP2A (arrow) was detected using the purified Pab.

Confocal immunofluorescent analysis of TOP2A Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Formalin-fixed and paraffin-embedded human skin with TOP2A Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-TOP2A Antibody (C-term) at 1:1000 dilution. Lane 1: Jurkat whole cell lysate. Lane 2: K562 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 174 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.