TOM1L2 Antibody

• Catalog Number: orb1246846
• Category: Antibodies
• Description: TOM1L2 Antibody
• Target: TOM1L2
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: CRKKPERSEDALFAL
• UniProt ID: Q6ZVM7
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 60kDa band observed in Human Fibroblast A431 lysates (calculated MW of 51.8kDa according to NP_653279). Recommended concentration: 0.1-1ug/ml.Immunohistochemistry:In paraffin embedded Human Skeletal Muscle shows variable staining from fibre to fibre. Recommended concentration, 5-10ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: TOM1L2, FLJ32746, target of myb1-like 2 (chicken),
• Research Area: Cancer Biology
• Note: For research use only
• NCBI: NP_001076437.1, NP_001028723.1

orb1246846 (0.03 ug/ml) staining of A431 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1246846 (5 ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.