TOM1L1/SRCASM Antibody

• Catalog Number: orb19785
• Category: Antibodies
• Description: Goat polyclonal antibody to TOM1L1
• Target: TOM1L1 / SRCASM
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: EQNKNQKEATNTTSE
• RRID: AB_10767500
• MW: 53
• Tested applications: ELISA, FC, IF, WB
• Dilution range: ELISA: 1:64000, WB: 0.3-1 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 48kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 53.0kDa according to NP_005477.1). Recommended concentration: 0.3-1 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti TOM1L1 antibody, anti SRCASM antibody, anti t
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 10040
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

1 ug/ml staining of A431 lysate 1 (A) + peptide (B), A431 lysate 2 (C) + peptide (D), HEK293 (E) + peptide (F), and HEL (G) + peptide (H) cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

1 µg/ml staining of Human Cerebellum lysate (35 µg protein in RIPA buffer). Detected by chemiluminecence.