TNRC6A Antibody

• Catalog Number: orb1257195
• Category: Antibodies
• Description: TNRC6A Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 950-1050 of human TNRC6A (NP_055309.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:1000IF: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 180kDa
• Target: TNRC6A
• UniProt ID: Q8NDV7
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: TNRC6A, CAGH26, DKFZp666E117, FLJ22043, GW1, GW182
• Note: For research use only
• Application notes: WB: 1:500 - 1:1000IF: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using TNRC6A antibody (orb1257195) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunofluorescence analysis of U2OS cells using TNRC6A antibody (orb1257195) at dilution of 1:100. Blue: DAPI for nuclear staining.