GW182 Rabbit Polyclonal Antibody

• Catalog Number: orb183979
• Category: Antibodies
• Description: GW182 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P
• Predicted Reactivity: Human, Mouse
• Reactivity: Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human GW182 (631-730/1962aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 210 kDa
• Target: TNRC6A
• UniProt ID: Q8NDV7
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: CAG repeat protein 26; CAGH26; DKFZp666E117; EDIE;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Dittmann, Klaus et al. New roles for nuclear EGFR in regulating the stability and translation of mRNAs associated with VEGF signaling PLoS One, 12, e0189087 (2017)

PubMed: 29253018

Applications

WB

Reactivity

Human

Paraformaldehyde-fixed, paraffin embedded Rat Stomach, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with GW182 Polyclonal Antibody, Unconjugated (orb183979) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.