TNNT1/TNT Antibody

SKU: orb1535112

Description

TNNT1/TNT Antibody

Images & Validation

• Tested Applications: ELISA, IHC, IHC-P, WB
• Dilution Range: IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:128000), WB (0.03 - 0.1 µg/ml)
• Reactivity: Human
• Application Notes:
  Further information: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Preliminary experiments gave an approx 50kD band in Human Skeletal Muscle lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 32.9kD according to NP_003274.3. The 50kD band was successfully blocked by incubation with the immunizing peptide.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-EKAQELSDWIHQLE, from the internal region of the protein sequence according to NP_003274.3NP_001119604.1NP_001119605.1.
• Target: TNNT1 / TNT
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Concentration: 0.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TNNT1, STNT, TNT, TNTS, Troponin-T1, skeletal, slow, ANM, Troponin T1, skeletal, slow, NEM5

Goat Anti-TNNT1 Antibody (0.03 µg/ml) staining of Human Skeletal Muscle lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.

Quick Database Links

Gene Symbol

TNNT1 / TNT