TNIK(S764) Antibody

• Catalog Number: orb1925367
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This TNIK(S764) antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between amino acids from the human region of human TNIK(S764).
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q9UKE5
• MW: 154943 Da
• Tested applications: IHC-P, WB
• Dilution range: WB: 1:500, WB: 1:500, IHC-P-Leica: 1:500
• Antibody Type: Primary Antibody
• Clone Number: RB59089
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: TRAF2 and NCK-interacting protein kinase, 2.7.11.1
• Note: For research use only

All lanes: Anti-TNIK (S764) Antibody at 1:500 dilution. Lane 1: Human brain lysate. Lane 2: Caco2 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 155 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-TNIK (S764) Antibody at 1:500 dilution. Lane 1: Human brain tissue lysate. Lane 2: Caco2 whole cell lysate. Lane 3: U-2 OS whole cell lysate. Lane 4: U-251 MG whole cell lysate. Lane 5: Mouse brain tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 155 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded human brain tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.