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Description
Research Area
Immunology & Inflammation
Images & Validation
−
| Tested Applications | FC, Functional Studies, IA, IHC-Fr, IP, WB |
|---|---|
| Dilution Range | For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For neutralization of biological activity in vitro dilutions have to be made according to the amount of TNF-alpha to be inactivated. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions. |
| Reactivity | Human |
Key Properties
−| Clonality | Monoclonal |
|---|---|
| Isotype | Mouse IgG1 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |

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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
IP
Immunoprecipitation
TNF-alpha, Human, mAb 4H31 Antibody (orb1109058)
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