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TMPO Antibody
SKU: orb1274464
Featured
Description
Research Area
Cell Biology, Signal Transduction
Images & Validation
−Item 1 of 5
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-410 of human TMPO (NP_001027454.1). |
| Target | TMPO |
| Molecular Weight | Observed: 51kDa/80kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Thymopoietin Antibody: TP, LAP2, CMD1T, LEMD4, PRO0868, Lamina-associated polypeptide 2, isoform alpha, Thymopoietin isoform alpha, TP alpha
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Western blot analysis of extracts of various cell lines, using TMPO antibody (orb1274464) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Western blot analysis of extracts from normal (control) and TMPO knockout (KO) 293T cells, using TMPO antibody (orb1274464) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
Immunohistochemistry of paraffin-embedded rat brain using TMPO Antibody (orb1274464) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using TMPO Antibody (orb1274464) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using TMPO Antibody (orb1274464) at dilution of 1:100 (40x lens).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
TMPO Antibody (orb1274464)
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