TMEPAI Antibody / PMEPA1

SKU: orb2642272

Description

PMEPA1 (prostate transmembrane protein, androgen induced 1), or TMEPAI (Transmembrane prostate androgen induced protein) is a 287 amino acid single-pass membrane protein that contains WW-binding motifs and localizes to the cell membrane. Expressed at high levels in prostate, kidney and ovary, PMEPA1/TMEPAI interacts with NEDD4 and may play a role in regulating AR (androgen receptor) levels, specifically in prostate cells. Down regulation of TMEPAI is observed in prostate tumors, suggesting that TMEPAI may exhibit activity as a tumor suppressor. Overexpression of this protein may play a role in multiple types of cancer.

Images & Validation

• Tested Applications: IHC-P
• Dilution range: The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Reactivity: Human
• Application Notes:
  Optimal dilution of the TMEPAI antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: PMEPA1/2696
• Immunogen: Full-length recombinant human protein was used as the immunogen for the TMEPAI antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

IHC staining of FFPE human prostate carcinoma with TMEPAI antibody (clone PMEPA1/2696). HIER: boil tissue sections in pH6, 10 mM citrate buffer, for 10-20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free TMEPAI antibody (clone PMEPA1/2696) as confirmation of integrity and purity.

IHC staining of FFPE human prostate carcinoma with TMEPAI antibody (clone PMEPA1/2696). HIER: boil tissue sections in pH6, 10 mM citrate buffer, for 10-20 min and allow to cool before testing.

IHC staining of FFPE human colon carcinoma with TMEPAI antibody (clone PMEPA1/2696). HIER: boil tissue sections in pH6, 10 mM citrate buffer, for 10-20 min and allow to cool before testing.

Quick Database Links

UniProt

Q969W9