TMEM150B Antibody (N-term)

• Catalog Number: orb1938225
• Category: Antibodies
• Description: TMEM150B Antibody (N-term)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: A6NC51
• MW: 25701 Da
• Tested applications: IF, IHC-P, WB
• Dilution range: WB - 1:1000, IHC-P - 1:100-500, IF - 1:10-50
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Research Area: Cell Biology
• Note: For research use only

All lanes: Anti-TMEM150B Antibody (N-term) at 1:1000 dilution. Lane 1: Caco2 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/15000 dilution.Observed band size: 36kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Confocal immunofluorescent analysis of TMEM150B Antibody (N-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

TMEM150B Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of TMEM150B Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

TMEM150B Antibody (N-term) western blot analysis in K562 cell line lysates (35 ug/lane). This demonstrates the TMEM150B antibody detected the TMEM150B protein (arrow).