TMBIM1 Antibody

SKU: orb344643

Description

TMBIM1 antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:10,000, IHC: 10µg/mL, WB: 1:500 - 1:2,000
• Reactivity: Human
• Application Notes:
  This protein A purified antibody has been tested for use in ELISA, Immunohistochemistry, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 35 kDa in size corresponding to TMBIM-1 by western blotting in the appropriate cell lysate or extract. To date this antibody has shown the ability to recognize over-expressed TMBIM1 but not endogenous protein.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to the amino terminus of human TMBIM1 protein.
• Purity: This product was protein A purified from monospecific antiserum by immunoaffinity chromatography using protein A coupled to agarose beads. This antibody is specific for human TMBIM1 protein. A BLAST analysis was used to suggest partial cross-reactivity with TMBIM1 from mouse and rat sources based on ~88% homology with the immunizing sequence. Cross-reactivity with TMBIM1 from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.2 mg/mL
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-TMBIM1 Antibody, Putative uncharacterized protein TMBIM1, Protein lifeguard 3, Protein RECS1 homolog, Transmembrane BAX inhibitor motif-containing protein 1, LFG3, RECS1, PP1201, PSEC0158

Immunohistochemistry of Rabbit anti-TMBIM1 antibody. Tissue: human prostate. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: anti-TMBIM1 antibody at 10 µg/ml for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10000 for 45 min at RT. Staining: TMBIM1 as precipitated red signal with hematoxylin purple nuclear counterstain.

Immunohistochemistry of Rabbit anti-TMBIM1 antibody. Tissue: human tonsil. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: anti-TMBIM1 antibody at 10 µg/ml for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10000 for 45 min at RT. Staining: TMBIM1 as precipitated red signal with hematoxylin purple nuclear counterstain.

Western blot using Biorbyt's protein A purified anti-TMBIM1 antibody shows detection of exogenous TMBIM1 in lysates from HeLa cells transfected with pcDNA3-hTMBIM1 (lane 1). No staining is observed in lysates from mock transformed HeLa cells (lane 2). To date this antibody has shown the ability to recognize over expressed TMBIM1 ~35kDa, but not endogenous protein. The membrane was probed with the primary antibody at a 1:1000 dilution at 4°C, overnight.

Quick Database Links

UniProt

Q969X1

RefSeq:50593008