TLR9, Human, mAb 5G5 Antibody

SKU: orb1108978

Description

Mouse monoclonal antibody to TLR9

Research Area

Cell Biology

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, PS, WB
• Dilution Range: For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
• Reactivity: Human
• Application Notes:
  F: 10 um sections were fixed with acetone for 10 minutes. PBS washed sections were incubated with 5G5 1:100 in 1% BSA for 30 minutes at RT. (Ref.7) IF: cells were fixed with 2% formalin for 15 minutes at RT and permeabilized with a mAb (4ug/400ul) containing buffer (PBS, 0.2% BSA, 0.2% saponin)- for 1 hour. (Ref.1) FC: RAW264.7 cells were fixed for 15 minutes with 4% formalin and permeabilized (PBS, 0.5%BSA, 0,5% saponin) at RT. (Ref.1) P: paraffin embedded tissues 5um;m sections were made. After antigen retrieval (0.01mol/l, pH6 sodium citrate) and quenching of endogenous peroxidase, sections were blocked with 0.5% ovalbumin and 0.1% gelatin for 20 minutes at RT. Sections were incubated with 5G5 for 1 hour at 37°C. (Ref.3) W: reduced lysates were resolved by 10% SDS-PAGE and blotted on nitrocellulose. After blocking with 5% skimmed milk TLR9 was detected with 2 µg/ml 5G5. (Ref.1)

Key Properties

• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Immunogen: Purified- fusion protein of extracellular domain of human TLR9 (AA 1-815) and human IgGFc
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1.0 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD289, TLR9

Quick Database Links

UniProt

Q9NR96