TLR9 Antibody

• Catalog Number: orb1538856
• Category: Antibodies
• Description: TLR9 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A peptide corresponding to 15 AA near the center of human TLR9. The immunogen is located within aa 840-890 of TLR9.
• Concentration: 1 mg/ml
• Dilution range: ELISA, ICC, IF, IHC, IHC-P (10 µg/ml), WB (0.5 - 1 µg/ml)
• Conjugation: Unconjugated
• Target: TLR9
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS containing 0.02% Sodium Azide. PBS containing 0.02% Sodium Azide
• Alternative names: TLR9, CD289, CD289 antigen, Scri2a, Toll-like rece
• Note: For research use only
• Application notes: Further information: TLR9 antibody can be used for detection of TLR9 by Western blot at 1 ug/mL. Antibody can also be used for immunocytochemistry starting at 2 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Western Blot: Predicted: 104, 111 kDa Observed: 97 kDa
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry of TLR9 in Jurkat cells with TLR9antibody at 2 ug/ml.

Immunofluorescence of TLR9 in Mouse Spleen cells with TLR9 antibody at 10 ug/ml.

Western blot of TLR9 in Jurkat cell lysate with TLR9 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.