TLR9 Antibody

• Catalog Number: orb1238751
• Category: Antibodies
• Description: TLR9 Antibody
• Target: TLR9
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1 mg/mL
• Buffer/Preservatives: TLR9 Antibody is supplied in PBS containing 0.02% sodium azide.
• Purification: TLR9 Antibody is affinity chromatography purified via peptide column.
• Immunogen: TLR9 antibody was raised against a peptide corresponding to 15 amino acids near the center of human TLR9.The immunogen is located within amino acids 840 - 890 of TLR9.
• UniProt ID: Q9NR96
• MW: Predicted: 104, 111 kDa Observed: 97 kDa
• Tested applications: ELISA, ICC, IF, WB
• Application notes: TLR9 antibody can be used for detection of TLR9 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: TLR9 Antibody: CD289, Toll-like receptor 9
• Research Area: Immunology & Inflammation, Signal Transduction
• Note: For research use only
• NCBI: AAH32713

Western blot analysis of TLR9 in Jurkat cell lysate with TLR9 antibody at 1 µg/mL in (A) the absence and (B) the presence of blocking peptide.

Immunocytochemistry of TLR9 in Jurkat cells with TLR9 antibody at 2 µg/mL.

Immunofluorescence of TLR9 in Mouse Spleen tissue with TLR9 antibody at 10 µg/mL.