TLR6 Rabbit Polyclonal Antibody

SKU: orb7096

Description

TLR6 Rabbit Polyclonal Antibody

Research Area

Defensins, Immune System, Inflammation, Innate and Adaptive Immunity, Macrophage, NF-kB Signalling Pathway, Signal Transduction, TLR Signaling Pathway

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=0.2μg /test
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Equine, Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human TLR6 (301-400/796aa)
• Target: TLR6
• Molecular Weight: 84 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD286; CD286 antigen; TLR 6; Toll like receptor 6 precursor; TLR6_HUMAN; Toll-like receptor 6.

Blank control: HUVEC. Primary Antibody (green line): Rabbit Anti-TLR6 antibody (orb7096), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control: Raji (blue). Primary Antibody: Rabbit Anti-TLR6 antibody (orb7096), dilution: 0.2 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. The cells were washed twice with phosphate-buffered saline (PBS). The cells were then incubated in 1X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the antibody (orb7096, 0.2 µg/1x10^6 cells) for 30 min on ice. The secondary antibody used was Goat Anti-rabbit IgG/PE antibody at 1/200 dilution for 30 min on ice. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (rat spleen tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TLR6) Polyclonal Antibody, Unconjugated (orb7096) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Rat splenocytes stained with Anti-TLR6/CD286 Polyclonal Antibody, PE-CY5.5 Conjugated at 1:25.

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-TLR6 Polyclonal Antibody, Unconjugated (orb7096) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

TLR6

UniProt

Q9Y2C9