TLR5 Antibody

• Catalog Number: orb1238759
• Category: Antibodies
• Description: TLR5 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: TLR5 antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human TLR5.The immunogen is located within the last 50 amino acids of TLR5.
• Concentration: 1 mg/mL
• Dilution range: TLR5 antibody can be used for detection of TLR5 by Western blot at 1 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 94 kDa Observed: 94 kDa
• Target: TLR5
• UniProt ID: O60602
• NCBI: NP_003259
• Storage: TLR5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: TLR5 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: TLR5 Antibody: TIL3, SLEB1, MELIOS, TIL3, Toll-lik
• Note: For research use only
• Application notes: TLR5 antibody can be used for detection of TLR5 by Western blot at 1 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of TLR5 expression in HT29 cell lysate with TLR5 antibody at (A) 1 and (B) 2 µg/ml.

Immunocytochemistry of TLR5 in THP-1 cells with TLR5 antibody at 10 µg/mL.

Immunofluorescence of TLR5 in THP1 cells with TLR5 antibody at 10 µg/mL.