Recombinant TLR4 Antibody

SKU: orb699508

Description

This MAb reacts with human Toll-like receptor 4 (TLR4). It is a member of the Toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness. Multiple transcript variants encoding different isoforms have been found for this gene.

Research Area

Epigenetics, Immunology, NF-kB, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: ELISA
• Dilution Range: ELISA: order Ab without BSA for coating
• Reactivity: Human
• Application Notes:
  Optimal dilution of the recombinant TLR4 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG
• Clone No.: TLR4/3895R
• Immunogen: Recombinant full-length human TLR4 protein was used as the immunogen for the recombinant TLR4 antibody.
• Purification: Protein A affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

SDS-PAGE analysis of purified, BSA-free TLR4 antibody (clone TLR4/3895R) as confirmation of integrity and purity.

Quick Database Links

UniProt

O00206