TLR2, Mouse, mAb T2.5 Antibody

SKU: orb1109144

Description

Mouse monoclonal antibody to TLR2

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC, Functional Studies, IA, IF, IHC-Fr, IP
• Dilution Range: Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.
• Reactivity: Human, Mouse
• Application Notes:
  IHC-F: 6um sections were fixed with aceton. Sections were blocked with goat serum and exposed o/n with T2.5. FC: 4*104 leukocytes/ml were stained for 30 minutes at 4°C. FS: mice were injected i.p. with 1 mg T2.5, after 1h incubation mice were challenged;T2.5 5 µg/ml was added to cell culture. IP: 40ug cleared protein was incubated with 2ug T2.5 for 1h at 4°C. IA: T2.5 as a detector.

Key Properties

• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Immunogen: Mouse TLR2 peptide
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 1 ml (100 µg/ml) 0.2 um filtered protein G purified antibody solution in PBS, containing 0.1% bovine serum albumin. The endotoxin concentration is < 24 EU/mg, determined with the LAL Assay.
• Buffer/Preservatives: Quantities >500 µg will be sent without preservatives and are carrier-free.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TLR2, CD282, TIL4 Gene name: Tlr2