TLK2 Antibody

SKU: orb1256785

Description

TLK2 Antibody

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human TLK2 (NP_006843.2).
• Target: TLK2
• Molecular Weight: Observed: 87kDa
• Purification: Affinity purification

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

Serine/threonine-protein kinase tousled-like 2, HsHPK, PKU-alpha, Tousled-like kinase 2, TLK2

Western blot analysis of extracts of various cell lines, using TLK2 antibody (orb1256785) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human liver damage using TLK2 antibody (orb1256785) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human esophageal cancer using TLK2 antibody (orb1256785) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using TLK2 antibody (orb1256785). Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

TLK2

UniProt

Q86UE8