TLK2 Antibody

• Catalog Number: orb1256785
• Category: Antibodies
• Description: TLK2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human TLK2 (NP_006843.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 87kDa
• Target: TLK2
• UniProt ID: Q86UE8
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: Serine/threonine-protein kinase tousled-like 2, Hs
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using TLK2 antibody (orb1256785) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human liver damage using TLK2 antibody (orb1256785) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human esophageal cancer using TLK2 antibody (orb1256785) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using TLK2 antibody (orb1256785). Blue: DAPI for nuclear staining.