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Catalog Number | orb345441 |
---|---|
Category | Antibodies |
Description | Tlk1 antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 730-755 of human Tlk1 (Tousled-like kinase) (isoform 1) protein. |
Concentration | 0.60 mg/mL |
Dilution range | ELISA: 1:10,000 - 1:50,000, WB: 1:200 - 1:2,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | This affinity purified antibody is directed against human Tlk-1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. This antibody reacts with both phosphorylated and non-phosphorylated forms of Tlk-1 at the S743 residue (isoform 1) of the protein. The antibody will react with all isoforms of Tlk-1. A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee, orangutan, and dog sources based on 100% homology for the immunogen sequence. Cross reactivity with Tlk-1 protein from mouse, rat and chicken may occur as this sequence only varies by one amino acid residue (93% homology). Cross reactivity with Tlk-1 homologues from other sources has not been determined. |
Conjugation | Unconjugated |
UniProt ID | Q9UKI8 |
NCBI | 33636698 |
Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-TLK1 antibody, TLK-1, TLK 1, PKU-beta Read more... |
Note | For research use only |
Application notes | This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 86 kDa, 89 kDa or 64 kDa in size corresponding to isoforms of TLk-1 by western blotting in the appropriate cell lysate or extract. This antibody reacts with both phosphorylated and non-phosphorylated forms of Tlk-1 at the S743 residue (isoform 1) of the protein. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of corresponding to isoform 3 of human Tlk-1 (arrowhead) using Tlk1 antibody
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, WB | |
Human, Mouse, Primate, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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