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TLE1 Antibody

Catalog Number: orb606650

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb606650
CategoryAntibodies
DescriptionKey players in the Notch pathway are the TLE genes, which are human homologs of the Drosophila groucho gene. Groucho is a transcriptional repressor that plays a key role in neurogenesis, segmentation and sex determination. Transducin-like enhancer protein 1 (TLE1) is a protein that is encoded by the TLE1 gene and is involved in control of hematopoiesis, neuronal, and terminal epithelial differentiation. Positive immunohistochemical nuclear staining with anti-TLE-1 has been shown to be a useful addition to an IHC panel when differentiating synovial sarcoma from other soft tissue malignancies.
Species/HostMouse
ClonalityMonoclonal
Clone NumberTLE1/2085
Tested applicationsIHC-P, WB
ReactivityHuman
IsotypeMouse IgG2a, kappa
ImmunogenA portion of amino acids 175-338 from the human protein was used as the immunogen for the TLE1 antibody.
Antibody TypePrimary Antibody
Dilution rangeWestern blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis TLE1 antibody is available for research use only.
UniProt IDQ04724
StorageStore the TLE1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesOptimal dilution of the TLE1 antibody should be determined by the researcher.
Expiration Date12 months from date of receipt.
TLE1 Antibody

Western blot testing of human HeLa cell lysate with TLE1 antibody (clone TLE1/2085). Predicted molecular weight ~83 kDa.

TLE1 Antibody

IHC testing of FFPE human endometrial carcinoma with TLE1 antibody (clone TLE1/2085). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

TLE1 Antibody

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using TLE1 antibody (clone TLE1/2085). These results demonstrate the foremost specificity of the TLE1/2085 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

TLE1 Antibody

SDS-PAGE analysis of purified, BSA-free TLE1 antibody (clone TLE1/2085) as confirmation of integrity and purity.

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