Alkaline Phosphatase Antibody (tissue-nonspecific)

SKU: orb386137

Description

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypo-phosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants, which encode the same protein, have been identified for this gene.

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the Alkaline Phosphatase antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: ALPL/597
• Immunogen: Recombinant human ALPL protein was used as the immunogen for the Alkaline Phosphatase antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC testing of FFPE human colon carcinoma with Alkaline Phosphatase antibody (ALPL/597). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human endometrial carcinoma with Alkaline Phosphatase antibody (ALPL/597). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human testicular carcinoma with Alkaline Phosphatase antibody (ALPL/597). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human ovarian carcinoma with Alkaline Phosphatase antibody (ALPL/597). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE analysis of purified, BSA-free Alkaline Phosphatase antibody (clone ALPL/597) as confirmation of integrity and purity.

Quick Database Links

UniProt

P05186