TIMP3 Antibody

• Catalog Number: orb1252693
• Category: Antibodies
• Description: TIMP3 Antibody
• Target: TIMP3
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Bovine, Canine, Equine, Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 0.2 mg/mL
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Purification: Protein A affinity chromatography
• Immunogen: Amino acids 175-211 of human TIMP3 Ab was used as the immunogen for this antibody (100% homologous in cow, dog and horse, and 94% homologous in mouse and rat).
• UniProt ID: P35625
• Tested applications: FC, IF, IHC, IP, WB
• Application notes: Flow Cytometry: use antibody at 0.5-1 ug/million cellsIF: 1-2 ug/mlWB: 0.5-1 ug/mlIP: 1-2ug/500ug protein lysateImmunohistochemistry (FFPE): 1-2 ug/ml for 30 minutes at RT (1)Prediluted format: incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TIMP3 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Metalloproteinase inhibitor 3, Protein MIG-5, Tiss
• Research Area: Cancer Biology, Cell Biology, Neuroscience, Signal
• Note: For research use only

IHC staining of human placenta tissue with TIMP3 antibody.