TIMP3 Antibody

SKU: orb1252693

Description

TIMP3 Antibody

Research Area

Cancer Biology, Cell Biology, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC, IP, WB
• Reactivity: Bovine, Canine, Equine, Human, Mouse, Rat
• Application Notes:
  Flow Cytometry: use antibody at 0.5-1 ug/million cellsIF: 1-2 µg/mLWB: 0.5-1 µg/mLIP: 1-2ug/500ug protein lysateImmunohistochemistry (FFPE): 1-2 µg/mL for 30 minutes at RT (1)Prediluted format: incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TIMP3 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Amino acids 175-211 of human TIMP3 Ab was used as the immunogen for this antibody (100% homologous in cow, dog and horse, and 94% homologous in mouse and rat).
• Target: TIMP3
• Purification: Protein A affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Metalloproteinase inhibitor 3, Protein MIG-5, Tissue inhibitor of metalloproteinases 3, TIMP-3, TIMP3

Quick Database Links

Gene Symbol

TIMP3

UniProt

P35625