TIMP3 Antibody

SKU: orb1252693

Description

TIMP3 Antibody

Research Area

Cancer Biology, Cell Biology, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC, IP, WB
• Reactivity: Bovine, Canine, Equine, Human, Mouse, Rat
• Application Notes:
  Flow Cytometry: use antibody at 0.5-1 ug/million cellsIF: 1-2 µg/mLWB: 0.5-1 µg/mLIP: 1-2ug/500ug protein lysateImmunohistochemistry (FFPE): 1-2 µg/mL for 30 minutes at RT (1)Prediluted format: incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TIMP3 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Amino acids 175-211 of human TIMP3 Ab was used as the immunogen for this antibody (100% homologous in cow, dog and horse, and 94% homologous in mouse and rat).
• Target: TIMP3
• Purification: Protein A affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Metalloproteinase inhibitor 3, Protein MIG-5, Tissue inhibitor of metalloproteinases 3, TIMP-3, TIMP3

IHC staining of human placenta tissue with TIMP3 antibody.

Quick Database Links

Gene Symbol

TIMP3

UniProt

P35625