Tissue Inhibitor of Metalloproteinase 1 Antibody / TIMP1

SKU: orb1822632

Description

TIMP-1, TIMP-2, TIMP-3 and TIMP-4 (for tissue inhibitor of metalloproteinases -1, -2, -3 and -4) complex with metalloproteinases such as collagenases, gelatinases and stromelysins, resulting in irreversible inactivation of the metalloproteinase. TIMP-1 is identical to EPA (erythroid-potentiation activity). PTH has been shown to be a regulator of TIMP-2 in osteoblastic cells. TIMP-3 may be involved in regulating trophoblastic invasion of the uterus as well as in regulating remodeling of the extracellular matrix during the folding of epithelia, and in the formation, branching and expansion of epithelial tubes. TIMP-4 is most highly expressed in heart tissues. Studies have demonstrated that TIMP1 is useful as a biomarker for early detection of colorectal cancer, outperforming CEA. Additionally, TIMP1 studies have demonstrated its role in CRC tumorigenesis, as well as observing its overexpression in metastatic lymph nodes.

Research Area

Cancer Biology; Musculoskeletal & Connective Tissue Research; Pharmacology & Drug Discovery

Images & Validation

• Tested Applications: ELISA, IHC-P
• Dilution Range: ELISA: 2-4mg/ml for coating (order BSA-free format),Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the Tissue Inhibitor of Metalloproteinase 1 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: TIMP1/4358
• Immunogen: A recombinant human protein fragment (within amino acids 1-200) was used as the immunogen for the Tissue Inhibitor of Metalloproteinase 1 antibody.
• Purification: Protein A affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

UniProt

P01033