TIMP1 Antibody

SKU: orb1539007

Description

TIMP1 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IHC, IHC-P, WB
• Dilution Range: ELISA (0.5 - 2 µg/ml), IHC, IHC-P (5 µg/ml), WB (0.1 - 0.2 µg/ml)
• Reactivity: Human
• Application Notes:
  Resuspension: Reconstitute with sterile water. Further information: This in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human TIMP-1 (3 ug/ml), a concentration of 5 ug/ml of this antibody is required. ELISA: To detect hTIMP-1 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human TIMP-1 as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTIMP-1. Western Blot: To detect hTIMP-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTIMP-1 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

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Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: E.coli derived recombinant Human TIMP-1 encoding aa 24-207
• Target: TIMP1
• Purification: Immunoaffinity purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Lyophilized from PBS, pH 7.2
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TIMP1, CLGI, EPA, HCI, Metalloproteinase inhibitor 1, TIMP-1

Quick Database Links

Gene Symbol

TIMP1