TIMP1 Antibody

• Catalog Number: orb1263706
• Category: Antibodies
• Description: TIMP1 Antibody
• Target: TIMP1
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
• Immunogen: This TIMP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-70 amino acids from the N-terminal region of human TIMP1.
• UniProt ID: P01033
• MW: 23 kDa
• Tested applications: FC, IF, IHC-P, WB
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50For IF starting dilution is: 1:10~50
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Metalloproteinase inhibitor 1, Erythroid-potentiat
• Research Area: Cancer Biology, Cell Biology, Signal Transduction
• Note: For research use only
• NCBI: P01033

Western blot analysis of TIMP1 Antibody in CEM cell line lysates (35 ug/lane)

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with TIMP1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Flow cytometric analysis of MDA-231 cells using TIMP1 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of TIMP1 Antibody with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).