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TIM-3 Antibody / HAVCR2

Catalog Number: orb699596

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb699596
CategoryAntibodies
DescriptionTIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk. TIM-3 is expressed on the surface of effector T cel tonsil, lymph node or spleen. ls (CD4+Th1 and CD8+Tc1) but not on helper T cells (CD4+Th2 and CD8+Tc2). In chronic inflammation, autoimmune disorders, and some cancers, TIM-3 is upregulated on several other hematopoietic cell types. The Ig domain of TIM-3 interacts with a ligand on resting but not activated Th1 and Th2 cells. The glycosylated Ig domain of TIM-3 binds cell-associated galectin-9. This induces TIM-3 Tyr phosphorylation and pro-apoptotic signaling. TIM-3 functions as a negative regulator of Th1 cell activity. Its blockade results in increased IFN-gamma production, Th1 cell proliferation and cytotoxicity, regulatory T cell development, and increases in macrophage and neutrophil infiltration into sites of inflammation.
Species/HostMouse
ClonalityMonoclonal
Clone NumberTIM3/4028
Tested applicationsIHC-P, WB
ReactivityHuman
IsotypeMouse IgG2c, kappa
ImmunogenA portion of amino acids 22-202 from the human protein was used as the immunogen for the TIM3 antibody.
Antibody TypePrimary Antibody
Dilution rangeWestern blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Hazard InformationThis TIM3 antibody is available for research use only.
UniProt IDQ8TDQ0
StorageStore the TIM3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
NoteFor research use only
Application notesOptimal dilution of the TIM3 antibody should be determined by the researcher.
Expiration Date12 months from date of receipt.
TIM-3 Antibody / HAVCR2

Western blot testing of human spleen lysate with TIM3 antibody (clone TIM3/4028). Expected molecular weight: 33-70 kDa depending on glycosylation level.

TIM-3 Antibody / HAVCR2

IHC staining of FFPE human lymph node with TIM3 antibody (clone TIM3/4028). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

TIM-3 Antibody / HAVCR2

IHC staining of FFPE human lymph node with TIM3 antibody (clone TIM3/4028). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

TIM-3 Antibody / HAVCR2

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using TIM3 antibody (clone TIM3/4028). These results demonstrate the foremost specificity of the TIM3/4028 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

TIM-3 Antibody / HAVCR2

SDS-PAGE analysis of purified, BSA-free TIM3 antibody (clone TIM3/4028) as confirmation of integrity and purity.

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