TIEG Antibody (N-term)

• Catalog Number: orb1928927
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB14876
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:2000, WB: 1:1000, IHC-P: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 52555 Da
• Target: This TIEG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-40 amino acids from the N-terminal region of human TIEG.
• UniProt ID: Q13118
• NCBI: NP_001027453.1, NP_005646.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Krueppel-like factor 10, EGR-alpha, Transforming g
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of anti-TIEG Antibody (N-term) pre-incubated with and without blocking peptide in CEM cell line lysate. TIEG (N-term) (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with TIEG antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-TIEG Antibody (N-term) at 1:2000 dilution. Lane 1: 293T whole cell lysates. Lane 2: MCF-7 whole cell lysates. Lane 3: human kidney lysates. Lane 4: human lung lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 53 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.