TIA1 Recombinant Rabbit Monoclonal Antibody

SKU: orb1173047

Description

TIA1 Recombinant Rabbit Monoclonal Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB=1:500-1000, Flow-Cyt=1:20-100
• Reactivity: Human
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: D1D5
• Target: TIA1
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ALS26; TIA-1; WDM; 2310050N03Rik; mTIA-1; TIA1_HUMAN; TIA1; Nucleolysin TIA-1 isoform p40; RNA-binding protein TIA-1; T-cell-restricted intracellular antigen-1 (TIA-1); p40-TIA-1; TIA1_MOUSE; Nucleolysin TIA-1; Tia;

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: HeLa, 2: Jurkat, 3: K562, 4: MOLT-4, 5: NIH-3T3, Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 43 kDa, Observed MW: 42-43 kDa.

Cell line: Jurkat, Fixative: 4% Paraformaldehyde, Permeabilization: 90% Methanol, Primary ab Dilution: 1:100, Secondary ab: Goat anti Rabbit IgG, Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Rabbit monoclonal IgG (Blue line). Comment: Line red is the positive signal for orb1173047.

Quick Database Links

Gene Symbol

TIA1