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Thyroid Hormone Receptor Antibody: Biotin

Catalog Number: orb612668

DispatchUsually dispatched within 2-3 weeks
$ 590.00
Catalog Numberorb612668
CategoryAntibodies
DescriptionMouse monoclonal antibody against NR1D1 conjugated to Biotin
TargetThyroid Hormone Receptor
ClonalityMonoclonal
Species/HostMouse
IsotypeIgG2a
ConjugationBiotin
ReactivityHuman, Mouse, Rat
Concentration1mg/ml
Buffer/Preservatives136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
ImmunogenSynthetic peptide from the full length Human Thyroid hormone receptor protein
UniProt IDP10827
MW70 kDa
Tested applicationsELISA, ICC, IF, IHC, WB
Dilution rangeWB (1:500); IHC (1:100)
Application notesA 1:500 dilution of SMC-561 was sufficient for detection of Pan-Thyroid hormone receptor in 10 ug of Hep G2 Human Hepatoblastoma Cell lysate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Clone NumberH43
StorageConjugated antibodies should be stored according to the product label
Alternative namesERBA ALPHA antibody, ERBA beta antibody, ERBA1 ant
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NoteFor research use only
Entrez7067
NCBINM_001190918.2
Thyroid Hormone Receptor Antibody: Biotin

Immunohistochemistry analysis using Mouse Anti-Thyroid Hormone Receptor Monoclonal Antibody, Clone H43. Tissue: Thyroid. Species: Mouse. Primary Antibody: Mouse Anti-Thyroid Hormone Receptor Monoclonal Antibody at 1:100 for Overnight at 4°C, then 30 min at 37°C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37°C. Counterstain: DAPI for 3 min at RT. Magnification: 7.5X.

Thyroid Hormone Receptor Antibody: Biotin

Immunohistochemistry analysis using Mouse Anti-Thyroid Hormone Receptor Monoclonal Antibody, Clone H43. Tissue: Thyroid Cancer. Species: Human. Primary Antibody: Mouse Anti-Thyroid Hormone Receptor Monoclonal Antibody at 1:100 for Overnight at 4°C, then 30 min at 37°C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37°C. Counterstain: DAPI for 3 min at RT. Magnification: 4X.

Thyroid Hormone Receptor Antibody: Biotin

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Thyroid Hormone Receptor Monoclonal Antibody, Clone H43. Tissue: Differentiated SH-SY5Y. Species: Human. Primary Antibody: Mouse Anti-Thyroid Hormone Receptor Monoclonal Antibody at 1:250. Secondary Antibody: AlexaFluor 488. Counterstain: phalloidin (Alexa 647, red), beta tubulin (Anti-beta III Tubulin Ab, Alexa 555, magenta) Hoechst (blue). (A) Phalloidin (B) Anti-beta III Tubulin Ab. (C) Thyroid Hormone Receptor Antibody. (D) Hoechst (E) Composite.

Thyroid Hormone Receptor Antibody: Biotin

Western Blot analysis of Human Hep G2 Hepatoblastoma Cell lysate showing detection of Thyroid Hormone Receptor protein using Mouse Anti-Thyroid Hormone Receptor Monoclonal Antibody, Clone H43. Load: 10 ug. Primary Antibody: Mouse Anti-Thyroid Hormone Receptor Monoclonal Antibody at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Other Band (s): Higher molceular weight bands could be due to PTMs.

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