Recombinant Thymidylate Synthase Antibody / TYMS

SKU: orb2636350

Description

It recognizes a protein of 36kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). It converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein has been reported to associate with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.

Images & Validation

• Tested Applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the recombinant Thymidylate Synthase antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG, kappa
• Clone No.: TYMS/9091R
• Immunogen: A recombinant human thymidylate synthase protein fragment (within amino acids 1-200) was used as the immunogen for the recombinant Thymidylate Synthase antibody.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

IHC staining of FFPE human prostate carcinoma tissue with recombinant Thymidylate Synthase antibody (clone TYMS/9091R). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

Negative control: IHC staining of FFPE human brain tissue using recombinant Thymidylate Synthase antibody (clone TYMS/9091R). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant Thymidylate Synthase antibody (clone TYMS/9091R) as confirmation of integrity and purity.

Quick Database Links

UniProt

P04818