Thymidine Phosphorylase Antibody / PD-ECGF

• Catalog Number: orb606692
• Category: Antibodies
• Description: Recognizes a 485 amino acid protein (55 kDa monomer / 110 kDa homodimer), identified as platelet-derived endothelial growth factor (PD-ECGF), also called Thymidine Phosphorylase (TP, Tymp) or Gliostatin. In the presence of inorganic orthophosphate, it catalyzes the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. Its enzymatic activity is crucial for angiogenic activity (metabolite is angiogenic). Higher levels of serum TP/PD-ECGF are observed in cancer patients. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites (e.g. 5 -deoxy-5-fluorouridine to 5-FU). High intra-cellular levels of TP/PD-ECGF are associated with increased chemosensitivity to such antimetabolites.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: TYMP/2890R
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: Human recombinant full length protein was used as the immunogen for this recombinant Thymidine Phosphorylase antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant Thymidine Phosphorylase antibody is available for research use only.
• UniProt ID: P19971
• Storage: Store the recombinant Thymidine Phosphorylase antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant Thymidine Phosphorylase antibody to be titered up or down for optimal performance.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human spleen tissue with recombinant Thymidine Phosphorylase antibody. Predicted molecular weight ~55 kDa.

SDS-PAGE analysis of purified, BSA-free recombinant Thymidine Phosphorylase antibody (clone TYMP/2890R) as confirmation of integrity and purity.

IHC staining of FFPE human prostate carcimoma with recombinant Thymidine Phosphorylase antibody (clone TYMP/2890R). Required HIER: requires boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min and allow to cool before testing.