You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1258271 |
---|---|
Category | Antibodies |
Description | THY1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 20-130 of human THY1 (NP_006279.2). |
Concentration | batch dependent |
Dilution range | WB: 1:1000 - 1:2000IF: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 25kDa |
Target | THY1 |
UniProt ID | P04216 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | Thy-1 membrane glycoprotein, CDw90, Thy-1 antigen, Read more... |
Note | For research use only |
Application notes | WB: 1:1000 - 1:2000IF: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using THY1 antibody (orb1258271) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Immunofluorescence analysis of Mouse brain cells using THY1 antibody (orb1258271) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of Rat brain cells using THY1 antibody (orb1258271) at dilution of 1:100. Blue: DAPI for nuclear staining.
ELISA, ICC, IF, IHC-P | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IHC-P, WB | |
Canine, Equine, Porcine, Sheep | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
Filter by Rating