Thrombospondin-1 Antibody / THBS1

SKU: orb1825434

Description

The thrombospondin proteins (TSP 1-4) compose a family of glycoproteins that are involved in cell-to-cell and cell-to-matrix signaling. These extracellular, cell surface proteins form complexes of both homo- and heteromultimers. Thrombospondins play a role in development, aggregation of platelets, adhesion and migration of cells, and progression of cells through the growth cycle. Thrombospondin 1 is released from platelets in response to Thrombin stimulation and is a transient component of the extracellular matrix of developing and repairing tissues. Thrombospondin 2 shares a high degree of homology with Thrombospondin 1 and is thought to have overlapping but unique functions. Thrombospondin 3 is a developmentally regulated heparin binding protein. Thrombospondin 4 is neuronally expressed and stimulates neurite outgrowth.

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IHC-P
• Dilution Range: ELISA: 2-4ug/ml for coating (order BSA-free format for coating),Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the Thrombospondin-1 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: THBS1/4532
• Immunogen: A recombinant partial protein sequence (within amino acids 200-400) from the human protein was used as the immunogen for the Thrombospondin-1 antibody.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

UniProt

P07996