Thiazovivin

SKU: orb1306302

Description

Thiazovivin is a potent ROCK inhibitor (IC50: 0.5 µM) that enhances human embryonic stem cell (hESC) survival. It is widely used in stem cell research to improve in vitro culture efficiency and maintain pluripotency, facilitating regenerative medicine studies.

Research Area

Stem Cell & Developmental Biology

Key Properties

• CAS Number: 1226056-71-8
• MW: 311.37
• Purity: > 98%
• Formula: C₁₅H₁₃N₅OS
• SMILES: O=C(NCc1ccccc1)c1csc(Nc2ccncn2)n1
• Target: Kinase
• Solubility: Soluble in DMSO (up to 30 mg/ml).

Bioactivity

• Target IC50:
  ROCK:~0.5 μM （cell free)
• In Vitro:
  When the cells were cultured in the presence of an E-cadherin blocking antibody, the formation of large, compact aggregates following Thiazovivin treatment was severely inhibited and extensive cell death was observed. Thiazovivin (2 μM) inhibits ROCK activity and protects hESCs at a similar level as Y-27632 (10 μM), a widely used selective ROCK inhibitor . Attachment rates of blastocyst and embryonic cell clumps onto feeder cells in the Thiazovivin treatment group were greater than those of the control group. The pluripotency markers of the OCT4 and NANOG genes and the adhesion molecule E-cadherin were increased by Thiazovivin treatment . By adding thiazovivin to reprogramming cultures, the reprogramming efficiency of CB cells increases by more than 10 times .
• Cell Research:
  Chemically defined and feeder-free human embryonic stem cell (hESC) culture was described briefly as following. hESCs were grown on Matrigel-coated tissue culture plates in N2B27-CDM [DMEM-F12 supplemented with 1× N2 supplements, 1× B27 supplements, 2 mM L glutamine, 0.11 mM 2-mercaptoethanol, 1× nonessential amino acids, and 0.5 mg/mL BSA (fraction V)] and 20 ng/mL bFGF. Human ESCs were passaged every five to six days with 0.05% trypsin. Murine ESCs are cultured in knockout DMEM supplement with 2 mM L glutamine, 1× nonessential amino acids, 15% serum replacement, and 1 × 10^3 ng/mL leukemia inhibitory factor (LIF). For clonal survival assays, single hESCs were diluted to clonal density and plated onto 96-well Matrigel-coated plate. For low-density survival assays, 500 cells were plated onto 96-well Matrigel-coated plate. To visualize hESC colonies, cultures were fixed in 4% paraformaldehyde in phosphate buffered saline (PBS) for 5 min, washed once in PBS, then stained for alkaline phosphatase (ALP) activity as described in the manufacturer's instructions. ALP-positive colonies were counted on an inverted microscope. For growing hESCs in mouse medium, HES2, HUES7, HUES9, and HUES1-Oct4-GFP were cultured in murine ESC (mESC) growth media supplemented with 1-μM mitogen-activated protein kinase/extracellular signal-regulated kinase kinase (MEK) inhibitor PD0325901 and 5-μM p38 inhibitor SB202190 and 1 × 10^3 human LIF .

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

inhibit, Inhibitor, Thiazovivin, Rho-associated kinase, Rho-associated protein kinase, Rho-kinase, ROK, ROCK

Compound Identifiers

PubChem CID

46209426