THBS1/Thrombospondin-1 Antibody

SKU: orb1538259

Description

THBS1/Thrombospondin-1 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IF, IHC, IHC-P, WB
• Dilution Range: IF (10 µg/ml), IHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:128000), WB (0.3 - 1 µg/ml)
• Reactivity: Bovine, Equine, Human, Monkey, Mouse, Porcine
• Application Notes:
  Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 3.75 ug/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence NRIPESGGDNSVFD-C, from the N-Terminus of protein sequence according to NP_003237.2.
• Target: THBS1 / Thrombospondin-1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Concentration: 0.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

THBS1, Thrombospondin-1, Thrombospondin-1p180, TSP, TSP1, THBS-1, THBS, Thrombospondin 1, TSP-1

THBS1 / Thrombospondin-1 antibody immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml).

THBS1 / Thrombospondin-1 antibody (1 µg/ml) staining of Mouse Kidney lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

THBS1 / Thrombospondin-1 antibody flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton.

Quick Database Links

Gene Symbol

THBS1 / Thrombospondin-1