THBS1 Antibody (N-term)

• Catalog Number: orb2649271
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB21031
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: IF: 1:10~50, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 129383 Da
• Target: This THBS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 181-210 amino acids from the N-terminal region of human THBS1.
• UniProt ID: P07996
• NCBI: NP_003237.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Thrombospondin-1, THBS1, TSP, TSP1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of THBS1 Antibody (N-term) in MDA-MB231 cell line lysates (35 ug/lane). THBS1 (arrow) was detected using the purified Pab.

Confocal immunofluorescent analysis of THBS1 Antibody (N-term) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

THBS1 Antibody (N-term) flow cytometric analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with THBS1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.