THAP1 Antibody

SKU: orb1256370

Description

THAP1 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-213 of human THAP1 (NP_060575.1).
• Target: THAP1
• Molecular Weight: Observed: 25kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DYT6, THAP domain-containing protein 1, 4833431A01Rik, THAP domain containing, apoptosis associated protein 1, THAP domain protein 1, nuclear proapoptotic factor

Western blot analysis of extracts of various cell lines, using THAP1 antibody (orb1256370) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded rat heart using THAP1 antibody (orb1256370) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using THAP1 antibody (orb1256370) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of A549 cells using THAP1 antibody (orb1256370).

Quick Database Links

Gene Symbol

THAP1

UniProt

Q9NVV9