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Item 1 of 4
TH Antibody (C-term)
Catalog Number: orb1929692
| Catalog Number | orb1929692 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB21096 |
| Tested applications | FC, IHC-P, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Dilution range | WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 58600 Da |
| Target | This TH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 486-514 amino acids from the C-terminal region of human TH. |
| UniProt ID | P07101 |
| NCBI | NP_000351.2, NP_954987.2, NP_954986.2 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Tyrosine 3-monooxygenase, Tyrosine 3-hydroxylase, Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Flow cytometric analysis of K562 cells using TH Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human brain tissue with TH Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
TH Antibody (C-term) western blot analysis in SH-SY5Y cell line lysates (35 ug/lane). This demonstrates the TH antibody detected the TH protein (arrow).
Western blot analysis of lysates from SW480, U-87 MG cell line (from left to right), using TH Antibody (C-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20 ug per lane.
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