TGM7 Antibody

• Catalog Number: orb1249004
• Category: Antibodies
• Description: TGM7 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Canine, Porcine
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: C-KIEISQGQGHSVT
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 75kDa band observed in Human Lung and Testis and in Mouse Kidney, Lung and Testis lysates (calculated MW of 79.9kDa according to Human NP_443187.1 and 79.8kDa according to Mouse NP_001153896.1). Recommended concentration: 0.1-0.3ug/ml. An additional band of unknown identity was also consistently observed at 17kDa. This band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: TGM7
• UniProt ID: Q96PF1
• NCBI: NP_443187.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: TGM7, transglutaminase 7, TGMZ, transglutaminase Z
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 75kDa band observed in Human Lung and Testis and in Mouse Kidney, Lung and Testis lysates (calculated MW of 79.9kDa according to Human NP_443187.1 and 79.8kDa according to Mouse NP_001153896.1). Recommended concentration: 0.1-0.3ug/ml. An additional band of unknown identity was also consistently observed at 17kDa. This band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249004 (0.1 ug/ml) staining of Mouse Kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1249004 (5 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.