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Item 1 of 3
TGM4 Antibody (Center)
Catalog Number: orb1929714
| Catalog Number | orb1929714 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB21271 |
| Tested applications | FC, IHC-P, WB |
| Reactivity | Human, Mouse |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000-1:2000, IHC-P: 1:50~100, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 77145 Da |
| Target | This TGM4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 135-164 amino acids from the Central region of human TGM4. |
| UniProt ID | P49221 |
| NCBI | NP_003232.2 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Protein-glutamine gamma-glutamyltransferase 4, Fib Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Flow cytometric analysis of CEM cells using TGM4 Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human prostate carcinoma with TGM4 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
All lanes: Anti-TGM4 Antibody (Center) at 1:1000-1:2000 dilution. Lane 1: PC-3 whole cell lysateLane 2: Hela whole cell lysate. Lane 3: LNCap whole cell lysate. Lane 4: CCRF-CEM whole cell lysate. Lane 5: SH-SY5Y whole cell lysate. Lane 6: Mouse prostate whole tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 77 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
